Elisa Kit for Progesterone
Introduction
Clinically progesterone measurement is of use in confirming occurrence of ovulation, in assessment of luteal function and to check the effectiveness of procedures for induction of ovulation. In the female, following the mid-cycle surge of LH, corpus luteum development begins, with an increase in the circulating levels of progesterone thus confirming ovulation followed by normal development and function of the corpus luteum. Maximum levels reached by 5 to 8 days after ovulation and decreased to follicular phase levels approximately 4 days before menstruation. If pregnancy takes place, progesterone levels remain elevated and increases progressively throughout pregnancy. Serum progesterone levels are also useful to evaluate patients at risk from spontaneous abortions in early pregnancy and for monitoring replacement therapy.
The enzyme-linked immunosorbant assay (ELISA) developed is a quantitative in-vitro diagnostic method for measuring Progesterone in serum. It is a simple two-hour ELISA method for direct measurement of progesterone in serum. ELISA kit based on our technology is quick, specific and sensitive. The major components of this test have been found stable at specific temperature for certain period.
This is a 2.5-hour assay requiring 50 m l serum. The kit is under evaluation for stability and performance and will be ready with in few months.
The cost of the kit will be around Rs. 1,500/- per 100-tube kit. Marketing costs will be extra.
Advantages of Indian technologies
- Low capital investment
- High employment potential
- Maximum use of local raw materials and manpower resources
- Adaptable levels of sophistication
Plant Parameters
Raw Material |
| Antibody-coated tubes, Assay Buffer |
| Substrate buffer, Substrate |
| Enzyme Conjugate, Biochemicals |
| Plant & Machinery |
| Elisa reader, Freezer, Ultracentrifuge |
| HPLC system, Colorimeter, Incubator |
| Polystyrene tubes, Microscope |
Economics |
| Plant & Machinery Rs. 10 lakh |
| The above figures are for budgetary purpose only and subject to change in subsequent offers. |
For further information please contact
National Research Development Corporation
( A Government of India Enterprise )
20-22, Zamroodhpur Community Center
Kailash Colony Extension
New Delhi 110 048. India
Ph: +91-11-26419904, 26417821, 26480767, 26432627
Fax: 011-26231877, 26460506, 26478010
Website: www.nrdcindia.com
E-mail: write2@nrdcindia.com
