Micropropagation Technology through Tissue Culture

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Introduction

Standardized protocols are available to distinguish hybrids from the of cotton, sunflower, castor, rice and sorghum using protein or isozyme and DNA markers.


Application

These techniques are applicable for testing genetic purity of hybrid seeds. The purity of CMS female parent can also be tested by RAPD analysis.


Process

Isoenzymes are extracted from 100 single seeds or seedling and analyzed by electrophoresis. DNA is extracted from 100 single seedlings and analyzed by RAPD using specific primers.


PARAMETERS

Testing hybrid purity  
Cotton Protein/isoenzyme
Caster Isoenzyme
Sunflower Isoenzyme
Sorghum Isoenzyme
rice RAPD

ManPower

Scientific 1
Technical 2
Skilled helper 2

RAW MATERIAL

Chemicals /Year .075
Glassware /Year .075
Miscellaneous /Year .05

PLANT AND MACHINERY

Power(kWhr) 80
Water(KI) 500
Fuel  

UTILITIES

Autoclave pH meter
Electronic Distillation
Balance Units
Hot Air Ovens Laminar flow
Timers Cultural Racks
Tube-Lights Gas Ovens
Generators Refrigerators
Pressure Cookers  

ECONOMICS

Plant and Machinery 3
Know how fees Nil
Consultancy fees .1
Operating Experience Proven

Advantages

  • Low capital investment
  • High employment potential
  • Maximum use of local raw materials and manpower resources
  • Adaptable levels of sophistication

For further information please contact :

Indian Council for Agricultural Research
Krishi Bhavan,
New Delhi-110001, India