Production of Recombinant Streptokinase

Introduction

Streptokinase, a thrombolytic drug, of great therapeutic value is produced commercially by E.coli. The method used by us depends on Bacillus Subtilis, which is a GRAS (Generally Regarded as Safe) bacteria. The drug will be produced by inserting gene for streptokinase in a high copy no. plasmid of Bacillus Subtilis, which will secrete it to the culture medium from where the protein drug will be purified (purification yet to be tried).

Special Features:

As mentioned above B.subtilis is a better & safer organism than E.coli. for production of therapeutic protein. Extracellular secretion by B.subtilis is an added advantage, as far as purification is concerned.

Prospective Users:

Pharmaceutical Industries

Keywords:

Recombinant Streptokinase, Bacillus Substilis

Type of Technology:

Process

For further information please contact :

The Director
Foundation for Innovation and Technology Transfer
Indian Institute of Technology Delhi
Hauz Khas, New Delhi-110016
Tel : 91-011-26597167, 26857762, 26581013
Fax : 91-011-26851169
E-mail : mdfitt@gmail.com