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Biphasic Medical Electroporator
Introduction
 This
instrument is a novel bio-medical electroporator for efficient,
easy and rapid delivery / insertion of bio-molecules, drugs
into in vitro and animal experimental systems for drug delivery,
transfection and gene therapy.
It operates by inducing Cell Electroporation, which is the
process of formation of transient micropores in cellular plasma
membrane by application of high intensity, short duration
electric field. Application of voltage beyond threshold causes
polarization of membrane components resulting in membrane
permeabilization for exogenous molecules like DNA and drugs.
The induced membrane permeability is reversible provided the
magnitude of generated electric fields do not exceed critical
limit, beyond which the cell gets irreversibly damaged. Induced
pores can remain open from seconds to minutes depending on
the type of cell and electric pulse.
Specification
| Mode |
|
Manual
: Single pulse of preset parameter
Auto : continuous
pulse of 1Hz/sec |
|
No. of pulses selectable
|
: |
1,2,4
and 8 |
| Burst freq.
|
: |
125Hz |
| Pulse distance
|
: |
8 msec |
| Pulse
duration |
: |
200
& 300 msec |
| Amplitude |
: |
±
450V |
| Output impedance
(Z) |
: |
400 W |
Applications
- Delivery of anti-tumor and anti- fungal drugs for cancer
and trans-dermal skin diseases therapy.
- Transfection of bacterial cells by DNA.
- Gene therapy of human normal and tumor cells.
- Electro-insertion of proteins /enzymes.
Contact for more information
Head,
Technology Transfer & Collaboration Division,
Bhabha Atomic Research Centre,
Trombay, Mumbai - 400 085
Tel : 091-022-25505337/25593897
Fax : 091-022-25505151
E-mail : headttcd@barc.gov.in
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