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Micropropagation Technology through
Tissue Culture
Standardized protocols are available
to distinguish hybrids from the of cotton, sunflower, castor,
rice and sorghum using protein or isozyme and DNA markers.
Application
These techniques are applicable for testing
genetic purity of hybrid seeds. The purity of CMS female parent
can also be tested by RAPD analysis.
Process
Isoenzymes are extracted from 100 single
seeds or seedling and analyzed by electrophoresis. DNA is
extracted from 100 single seedlings and analyzed by RAPD using
specific primers.
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Testing hybrid purity
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Cotton
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Protein/isoenzyme
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Caster
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Isoenzyme
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Sunflower
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Isoenzyme
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Sorghum
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Isoenzyme
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rice
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RAPD
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MANPOWER
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Scientific
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1
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Technical
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2
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Skilled helper
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2
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RAW MATERIAL
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Chemicals /Year
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.075
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Glassware /Year
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.075
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Miscellaneous /Year
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.05
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PLANT AND MACHINERY
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Power(kWhr)
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80
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Water(KI)
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500
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Fuel
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UTILITIES
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Autoclave
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pH meter
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Electronic
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Distillation
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Balance
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Units
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Hot Air Ovens
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Laminar flow
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Timers
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Cultural Racks
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Tube-Lights
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Gas Ovens
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Generators
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Refrigerators
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Pressure Cookers
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ECONOMICS
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Plant and Machinery
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3
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Know how fees
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Nil
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Consultancy fees
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.1
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Operating Experience
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Proven
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Advantages
- Low capital investment
- High employment potential
- Maximum use of local raw materials and
manpower resources
- Adaptable levels of sophistication
For More Information, Contact:
Indian Council for Agricultural Research
Krishi Bhavan,
New Delhi-110001, India
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