Technologies in Health
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Biphasic Medical Electroporator
Introduction
This
instrument is a novel bio-medical electroporator for
efficient, easy and rapid delivery / insertion of bio-molecules,
drugs into in vitro and animal experimental systems
for drug delivery, transfection and gene therapy.
It operates by inducing Cell Electroporation, which
is the process of formation of transient micropores
in cellular plasma membrane by application of high intensity,
short duration electric field. Application of voltage
beyond threshold causes polarization of membrane components
resulting in membrane permeabilization for exogenous
molecules like DNA and drugs. The induced membrane permeability
is reversible provided the magnitude of generated electric
fields do not exceed critical limit, beyond which the
cell gets irreversibly damaged. Induced pores can remain
open from seconds to minutes depending on the type of
cell and electric pulse.
Specification
Mode
No. of pulses selectable
Burst freq.
Pulse distance
Pulse duration
Amplitude
Output impedance (Z)
:
:
:
:
:
:
:
Manual
: Single pulse of preset parameter
Auto :
continuous pulse of 1Hz/sec
1,2,4 and 8
125Hz
8 msec
200 & 300 msec
± 450V
400 W
Applications
Delivery of anti-tumor and
anti- fungal drugs for cancer and trans-dermal skin
diseases therapy.
Transfection of bacterial cells
by DNA.
Gene therapy of human normal and
tumor cells.
Electro-insertion of proteins
/enzymes.
Contact for
more information Head,
Technology Transfer & Collaboration Division,
Bhabha Atomic Research Centre,
Trombay, Mumbai - 400 085
Tel : 091-022-25505337/25593897
Fax : 091-022-25505151
E-mail : headttcd@barc.gov.in
This
instrument is a novel bio-medical electroporator for
efficient, easy and rapid delivery / insertion of bio-molecules,
drugs into in vitro and animal experimental systems
for drug delivery, transfection and gene therapy.